Importing data

Note

For users conducting the “Quick-start”, please refer to the steps below to create a collection of your input FASTQ files

• Upload all your fastq files using the “Upload data” on the left upper-hand corner on the Galaxy homepage (If your file is above 10GB, please use these instructions)

• These files should appear in your history on the right-hand side. Click on the checkbox and a checkbox will appear in front of every file

• Check all those that you would like to include in your analysis

• Click “For all selected” and choose “Build List of Dataset Pairs”

• In the new panel that appears, rename the text of unpaired forward to a common selector for forward reads and do the same for reverse reads (For example, if your forward reads have _R1 and reverse reads have _R2, use _R1 for selecting forward reads and _R2 for reverse reads)

• Click “Pair these datasets” for each valid forward and reverse pair

• Enter a name for the collection and click “Create List” to build the collection

• To lock the changes, click on the checkmark icon at the top of the history

• Your collection of fastq files is ready to be used in the workflow